Hotspot

trnG-UCC-trnS-GCU

Genome · 54860-55675 · 816 bp

Back to Wisteria

Pi 0.0252
MarkerSeek score 74.40
Primer available yes
Label trnG-UCC-trnS-GCU

Feature Metadata

label_name
trnG-UCC-trnS-GCU
feature_type
igs
region
Genome
length_bp
816
variable_sites
20
pi
0.0252
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnS-GCU_p1 TGAATCAAGCAAGGGATCCC CAATCCGACGCTTTAGTCCA 50.0 50.0 57.6 58.0 928-937 bp 1.0 79.9
trnG-UCC-trnS-GCU_p2 TGAATCAAGCAAGGGATCCC AATCCGACGCTTTAGTCCAC 50.0 50.0 57.6 58.0 927-936 bp 1.0 79.9
trnG-UCC-trnS-GCU_p3 TGAATCAAGCAAGGGATCCC ACGGAAAGAGAGGGATTCGA 50.0 50.0 57.6 58.2 981-990 bp 1.0 78.6
trnG-UCC-trnS-GCU_p4 TGAATCAAGCAAGGGATCCC AGCAATCCGACGCTTTAGTC 50.0 50.0 57.6 58.4 930-939 bp 1.0 77.1
trnG-UCC-trnS-GCU_p5 TGAATCAAGCAAGGGATCCC CGACGCTTTAGTCCACTCAG 50.0 55.0 57.6 58.4 923-932 bp 1.0 76.9