Hotspot

atpH-atpF

Genome · 49994-50659 · 666 bp

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Pi 0.00901

MarkerSeek score 63.90

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: Genome
length_bp: 666
variable_sites: 6
pi: 0.00901
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	CCGGAAGCAGAGGGAAAAAT	GATGGCCAGTAACTCAAGCA	50.0	50.0	58.2	57.9	833-834 bp	1.0	78.4
atpH-atpF_p2	CCGGAAGCAGAGGGAAAAAT	CGGATGGCCAGTAACTCAAG	50.0	55.0	58.2	58.3	835-836 bp	1.0	76.5
atpH-atpF_p3	CCGGAAGCAGAGGGAAAAAT	TGGCCAGTAACTCAAGCAAA	50.0	45.0	58.2	57.6	831-832 bp	1.0	76.4
atpH-atpF_p4	TGGGCTGGTTGTAGCATTAG	GATGGCCAGTAACTCAAGCA	50.0	50.0	57.6	57.9	762-763 bp	1.0	76.4
atpH-atpF_p5	AGCAGAGGGAAAAATACGGG	GATGGCCAGTAACTCAAGCA	50.0	50.0	57.6	57.9	828-829 bp	1.0	76.3