Hotspot

trnY-GUA-trnD-GUC

Genome · 32186-32623 · 438 bp

Back to Wisteria

Pi 0.0161

MarkerSeek score 65.50

Primer available yes

Label trnY-GUA-trnD-GUC

Feature Metadata

label_name: trnY-GUA-trnD-GUC
feature_type: igs
region: Genome
length_bp: 438
variable_sites: 7
pi: 0.0161
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnD-GUC_p1	TGGCAATATGTCTACGCTGG	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.8	58.5	508-546 bp	1.0	75.9
trnY-GUA-trnD-GUC_p2	GGCAATATGTCTACGCTGGT	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.8	58.5	507-545 bp	1.0	75.9
trnY-GUA-trnD-GUC_p3	GGGGACGGACTGTAAATTCG	CGGTGCTCTGACCAATTGAA	55.0	50.0	58.4	58.5	529-567 bp	1.0	75.0
trnY-GUA-trnD-GUC_p4	GGGACGGACTGTAAATTCGT	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.6	58.5	528-566 bp	1.0	74.8
trnY-GUA-trnD-GUC_p5	GATTCTTCCTGGGTCGATGC	CGGTGCTCTGACCAATTGAA	55.0	50.0	58.4	58.5	562-600 bp	1.0	74.4