Hotspot

trnK-UUU-rbcL

Genome · 4491-5528 · 1038 bp

Back to Wisteria

Pi 0.00491

MarkerSeek score 63.30

Primer available yes

Label trnK-UUU-rbcL

Feature Metadata

label_name: trnK-UUU-rbcL
feature_type: igs
region: Genome
length_bp: 1038
variable_sites: 5
pi: 0.00491
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	TGGAGTCGCACTTAAAAGCC	AGTCCTGAATTAGCTGCTGC	50.0	50.0	58.5	58.0	1155-1161 bp	1.0	77.5
trnK-UUU-rbcL_p2	AACCCTACCGATGGTATGGA	AGTCCTGAATTAGCTGCTGC	50.0	50.0	57.5	58.0	1208-1214 bp	1.0	77.4
trnK-UUU-rbcL_p3	AGTCGTGGTCTTACAAACCC	AGTCCTGAATTAGCTGCTGC	50.0	50.0	57.5	58.0	1223-1229 bp	1.0	77.0
trnK-UUU-rbcL_p4	GTCGTGGTCTTACAAACCCT	AGTCCTGAATTAGCTGCTGC	50.0	50.0	57.5	58.0	1222-1228 bp	1.0	77.0
trnK-UUU-rbcL_p5	AGTCGCACTTAAAAGCCGAG	AGTCCTGAATTAGCTGCTGC	50.0	50.0	58.6	58.0	1152-1158 bp	1.0	76.8