Hotspot

rpl32

SSC · 131421-131579 · 159 bp

Back to Wikstroemia

Pi 0.0478

MarkerSeek score 76.00

Primer available yes

Label rpl32

Feature Metadata

label_name: rpl32
feature_type: gene
region: SSC
length_bp: 159
variable_sites: 31
pi: 0.0478
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32_p1	AAAGACCACATACGCCGAAG	TAGGAATAGGCGATGGGGAA	50.0	50.0	58.3	57.6	863-902 bp	1.0	86.5
rpl32_p2	AAAAGACCACATACGCCGAA	TAGGAATAGGCGATGGGGAA	45.0	50.0	57.8	57.6	864-903 bp	0.9	83.3
rpl32_p3	TGGGAAAAGACCACATACGC	TAGGAATAGGCGATGGGGAA	50.0	50.0	58.2	57.6	868-907 bp	0.9	83.3
rpl32_p4	AAAAGACCACATACGCCGAA	AGGAATAGGCGATGGGGAAA	45.0	50.0	57.8	58.5	863-902 bp	0.9	82.8
rpl32_p5	TGGGAAAAGACCACATACGC	AGGAATAGGCGATGGGGAAA	50.0	50.0	58.2	58.5	867-906 bp	0.9	82.7