Hotspot

ndhF-rpl32

SSC · 130977-131420 · 444 bp

Back to Wikstroemia

Pi 0.0642

MarkerSeek score 83.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 444
variable_sites: 96
pi: 0.0642
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACACATTCCAACCAGTTCCC	AAAAAGCTTTCAAGGCTGCC	50.0	45.0	58.0	57.8	997-1042 bp	0.9	86.0
ndhF-rpl32_p2	CACATTCCAACCAGTTCCCA	AAAAAGCTTTCAAGGCTGCC	50.0	45.0	58.0	57.8	996-1041 bp	0.9	86.0
ndhF-rpl32_p3	ACACATTCCAACCAGTTCCC	GCTGCCCAATACCCTTTCTT	50.0	50.0	58.0	58.1	993-1028 bp	0.5	69.0
ndhF-rpl32_p4	CACATTCCAACCAGTTCCCA	GCTGCCCAATACCCTTTCTT	50.0	50.0	58.0	58.1	992-1027 bp	0.5	69.0
ndhF-rpl32_p5	AGATTTAGCAACTGCCCCAC	GCTGCCCAATACCCTTTCTT	50.0	50.0	58.2	58.1	1251-1286 bp	0.5	66.3