Hotspot

rps18-rpl20

LSC · 71739-72099 · 361 bp

Back to Wikstroemia

Pi 0.0232
MarkerSeek score 71.50
Primer available yes
Label rps18-rpl20

Feature Metadata

label_name
rps18-rpl20
feature_type
igs
region
LSC
length_bp
361
variable_sites
30
pi
0.0232
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.98
markerseek_score
71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps18-rpl20_p1 GAAAGAGGGGAATCGGTCAC GCAGTTGCTTCTTAATCGTA 55.0 40.0 58.0 53.8 460-497 bp 1.0 55.2
rps18-rpl20_p2 GAGGGGAATCGGTCACTAGA GCAGTTGCTTCTTAATCGTA 55.0 40.0 57.9 53.8 456-493 bp 1.0 54.9
rps18-rpl20_p3 AGAGGGGAATCGGTCACTAG GCAGTTGCTTCTTAATCGTA 55.0 40.0 57.9 53.8 457-494 bp 1.0 54.8
rps18-rpl20_p4 GAAAGAGGGGAATCGGTCAC GCAGTTGCTTCTTAATCGTAA 55.0 38.1 58.0 54.6 460-497 bp 1.0 54.1
rps18-rpl20_p5 GAGGGGAATCGGTCACTAGA GCAGTTGCTTCTTAATCGTAA 55.0 38.1 57.9 54.6 456-493 bp 1.0 53.8