Hotspot

petA-psbJ

LSC · 66099-66887 · 789 bp

Back to Wikstroemia

Pi 0.0299

MarkerSeek score 80.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 789
variable_sites: 77
pi: 0.0299
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTTCAAGGCCTTTTGT	TGGTTCATATTCTGGATTGGGT	45.0	40.9	58.5	57.1	878-938 bp	1.0	63.5
petA-psbJ_p2	CCATTACGTGTTCAAGGCCT	TGGTTCATATTCTGGATTGGGT	50.0	40.9	58.2	57.1	883-943 bp	0.9	61.9
petA-psbJ_p3	CCATTACGTGTTCAAGGCCT	ATTCTGGATTGGGTTCATCC	50.0	45.0	58.2	55.1	875-935 bp	0.9	61.4
petA-psbJ_p4	ACCCATTACGTGTTCAAGGC	TGGTTCATATTCTGGATTGGGT	50.0	40.9	58.5	57.1	885-945 bp	0.9	59.5
petA-psbJ_p5	CCATTACGTGTTCAAGGCCT	TGGTTCATATTCTGGATTGGGTT	50.0	39.1	58.2	57.8	883-943 bp	0.9	59.3