Hotspot

ndhK-ndhC

LSC · 53103-53173 · 71 bp

Back to Wikstroemia

Pi 0.0448

MarkerSeek score 73.30

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 71
variable_sites: 10
pi: 0.0448
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	GGGTCTGTCGATCAAGTAAGG	CATGGCGAAAAGGAGCATTG	52.4	50.0	57.8	58.1	134-151 bp	1.0	80.8
ndhK-ndhC_p2	GGTCTGTCGATCAAGTAAGGG	CATGGCGAAAAGGAGCATTG	52.4	50.0	57.8	58.1	133-150 bp	1.0	80.8
ndhK-ndhC_p3	TGGGTCTGTCGATCAAGTAAG	CATGGCGAAAAGGAGCATTG	47.6	50.0	57.1	58.1	135-152 bp	1.0	75.1
ndhK-ndhC_p4	GGGTCTGTCGATCAAGTAAGG	GCGAAAAGGAGCATTGGAAT	52.4	45.0	57.8	57.1	130-147 bp	1.0	74.1
ndhK-ndhC_p5	GGTCTGTCGATCAAGTAAGGG	GCGAAAAGGAGCATTGGAAT	52.4	45.0	57.8	57.1	129-146 bp	1.0	74.1