Hotspot

rps4-trnT-UGU

LSC · 49132-49672 · 541 bp

Back to Wikstroemia

Pi 0.018

MarkerSeek score 65.70

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 541
variable_sites: 34
pi: 0.018
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TAAGATCGCTTCCGGCTCTA	GCCCCTTTTGATTTCTTGCC	50.0	50.0	58.0	57.9	1318-1504 bp	1.0	88.8
rps4-trnT-UGU_p2	TAAGATCGCTTCCGGCTCTA	GCGGCAATTTGGTGGAATAC	50.0	50.0	58.0	58.1	1171-1339 bp	0.8	79.6
rps4-trnT-UGU_p3	GGGAGGGGTGCAAATTTGTA	GCGGCAATTTGGTGGAATAC	50.0	50.0	58.1	58.1	1730-1898 bp	0.8	78.6
rps4-trnT-UGU_p4	TTTCTAAGATCGCTTCCGGC	GCGGCAATTTGGTGGAATAC	50.0	50.0	58.1	58.1	1175-1343 bp	0.7	74.7
rps4-trnT-UGU_p5	TAAGATCGCTTCCGGCTCTA	CTCTACCGTGGACCCCTATT	50.0	55.0	58.0	57.9	1285-1314 bp	0.2	49.2