Hotspot

psaA-ycf3

LSC · 44502-45337 · 836 bp

Back to Wikstroemia

Pi 0.0151

MarkerSeek score 78.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 836
variable_sites: 51
pi: 0.0151
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	GCCGCTGAATATTGGAAACG	50.0	50.0	57.9	57.9	863-1012 bp	1.0	86.4
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	ATTGGAAACGGGCTATAGCG	50.0	50.0	57.9	58.1	853-1002 bp	1.0	86.4
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	ATAGCGCTTACTCCTGGGAA	50.0	50.0	57.9	58.2	839-988 bp	1.0	85.7
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TAGCGCTTACTCCTGGGAAT	50.0	50.0	57.9	58.2	838-987 bp	1.0	85.7
psaA-ycf3_p5	GAGAAATGACCCGGTCTAGC	GCCGCTGAATATTGGAAACG	55.0	50.0	57.8	57.9	881-1030 bp	1.0	85.5