Hotspot

atpH-atpI

LSC · 14720-15917 · 1198 bp

Back to Wikstroemia

Pi 0.0195

MarkerSeek score 71.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1198
variable_sites: 92
pi: 0.0195
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1174-1304 bp	1.0	88.5
atpH-atpI_p2	AGCGGCAGAAATCAATGGAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	57.9	57.9	1166-1296 bp	1.0	88.3
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	1144-1274 bp	1.0	88.3
atpH-atpI_p4	AGCGGCAGAAATCAATGGAT	CGAATCCATGGAGGGTCATC	45.0	55.0	57.9	57.8	1136-1266 bp	1.0	88.1
atpH-atpI_p5	AAGCGGCAGAAATCAATGGA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	1167-1297 bp	1.0	87.5