Hotspot

psbK-psbI

LSC · 8118-8552 · 435 bp

Back to Wikstroemia

Pi 0.0265

MarkerSeek score 71.60

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 435
variable_sites: 41
pi: 0.0265
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CAAGCCGCTGTAAGTTTTCG	ACAAAGAGTTTGAGAGTAAGCA	50.0	36.4	58.0	55.6	438-485 bp	1.0	55.4
psbK-psbI_p2	AGCCGCTGTAAGTTTTCGAT	ACAAAGAGTTTGAGAGTAAGCA	45.0	36.4	57.9	55.6	436-483 bp	1.0	55.0
psbK-psbI_p3	AAGCCGCTGTAAGTTTTCGA	ACAAAGAGTTTGAGAGTAAGCA	45.0	36.4	58.1	55.6	437-484 bp	1.0	54.8
psbK-psbI_p4	GCCGCTGTAAGTTTTCGATG	ACAAAGAGTTTGAGAGTAAGCA	50.0	36.4	57.8	55.6	435-482 bp	1.0	54.5
psbK-psbI_p5	GCAAGCCGCTGTAAGTTTTC	ACAAAGAGTTTGAGAGTAAGCA	50.0	36.4	58.3	55.6	439-486 bp	1.0	53.2