Hotspot

trnK-UUU-rps16

LSC · 4334-5023 · 690 bp

Back to Wikstroemia

Pi 0.0272

MarkerSeek score 70.70

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 690
variable_sites: 68
pi: 0.0272
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	TCGATCCCGAAGAGAAGGAA	50.0	50.0	58.2	57.9	886-908 bp	1.0	88.0
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	TCGATCCCGAAGAGAAGGAA	50.0	50.0	58.2	57.9	885-907 bp	1.0	88.0
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	TCGTTGCAGTTGATGTTCGA	50.0	45.0	58.2	58.1	901-923 bp	0.9	83.2
trnK-UUU-rps16_p4	AAAGCCGAGTACTCTACCGT	GTCCCATGGATTTGCTGGAT	50.0	50.0	58.2	57.9	1084-1106 bp	0.8	78.4
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	GTCCCATGGATTTGCTGGAT	50.0	50.0	58.2	57.9	1083-1105 bp	0.8	78.4