Hotspot

psbA

Genome · 1-1062 · 1062 bp

Back to Widdringtonia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: Genome
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TGGCCAAGCAGCTAAAAAGA	TCCCTACCCTATTGACTGCA	45.0	50.0	58.0	57.4	725 bp	1.0	75.5
psbA_p2	TGGCCAAGCAGCTAAAAAGA	ACCCTATTGACTGCAACCTC	45.0	50.0	58.0	57.2	720 bp	1.0	73.6
psbA_p3	ACTGGCCAAGCAGCTAAAAA	TCCCTACCCTATTGACTGCA	45.0	50.0	58.3	57.4	727 bp	1.0	73.1
psbA_p4	GCCATAGTGCTAATGCCCAA	TCCCTACCCTATTGACTGCA	50.0	50.0	58.3	57.4	769 bp	1.0	73.0
psbA_p5	CTATTACTGGCCAAGCAGCT	TCCCTACCCTATTGACTGCA	50.0	50.0	57.7	57.4	732 bp	1.0	72.8