Hotspot

trnD-GUC-psbM

Genome · 21506-22185 · 680 bp

Back to Widdringtonia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: Genome
length_bp: 680
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TTAGAGTACCGCCCTGTCAA	TGAGGCTTCGTTTGTGCATA	50.0	45.0	58.1	57.8	830 bp	1.0	78.0
trnD-GUC-psbM_p2	ATTGGTTAGAGTACCGCCCT	TGAGGCTTCGTTTGTGCATA	50.0	45.0	58.1	57.8	835 bp	1.0	77.6
trnD-GUC-psbM_p3	TTAGAGTACCGCCCTGTCAA	CTTTGAGGCTTCGTTTGTGC	50.0	50.0	58.1	58.2	833 bp	1.0	77.5
trnD-GUC-psbM_p4	TGTTTTACACGACGCAGCTA	TGAGGCTTCGTTTGTGCATA	45.0	45.0	57.9	57.8	895 bp	1.0	77.5
trnD-GUC-psbM_p5	ATTGGTTAGAGTACCGCCCT	CTTTGAGGCTTCGTTTGTGC	50.0	50.0	58.1	58.2	838 bp	1.0	77.1