Hotspot

psbD-trnT-GGU

Genome · 19851-20818 · 968 bp

Back to Widdringtonia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: Genome
length_bp: 968
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	AATAAACCGGACCAACCCAC	GTAACGCCATGGTAAGACGT	50.0	50.0	58.1	58.0	1128 bp	1.0	79.3
psbD-trnT-GGU_p2	CAATAAACCGGACCAACCCA	GTAACGCCATGGTAAGACGT	50.0	50.0	58.1	58.0	1129 bp	1.0	79.3
psbD-trnT-GGU_p3	ACAAAACGGTCTCTGCGTAG	GTAACGCCATGGTAAGACGT	50.0	50.0	58.2	58.0	1104 bp	1.0	78.2
psbD-trnT-GGU_p4	AACAAAACGGTCTCTGCGTA	GTAACGCCATGGTAAGACGT	45.0	50.0	57.8	58.0	1105 bp	1.0	78.2
psbD-trnT-GGU_p5	CGTAGCCAGTCATCCACAAT	GTAACGCCATGGTAAGACGT	50.0	50.0	57.7	58.0	1089 bp	1.0	77.5