Hotspot

matK-trnK-UUU

Genome · 3243-3884 · 642 bp

Back to Widdringtonia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: Genome
length_bp: 642
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TGCGTAAAGATCTTCCCGAA	TCAATGGTAGAGTACTCGGCT	45.0	47.6	57.0	58.0	752 bp	1.0	63.5
matK-trnK-UUU_p2	TTGCGTAAAGATCTTCCCGA	TCAATGGTAGAGTACTCGGCT	45.0	47.6	57.0	58.0	753 bp	1.0	63.5
matK-trnK-UUU_p3	TGCGTAAAGATCTTCCCGAA	AGGGTTGCTAACTCAATGGT	45.0	45.0	57.0	56.7	764 bp	1.0	61.5
matK-trnK-UUU_p4	TTGCGTAAAGATCTTCCCGA	AGGGTTGCTAACTCAATGGT	45.0	45.0	57.0	56.7	765 bp	1.0	61.5
matK-trnK-UUU_p5	TGCGTAAAGATCTTCCCGAAA	TCAATGGTAGAGTACTCGGCT	42.9	47.6	57.7	58.0	752 bp	1.0	61.3