Hotspot

psbA

LSC · 45156-46217 · 1062 bp

Back to Werauhia

Pi 0.000942

MarkerSeek score 48.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 1
pi: 0.000942
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	CAAGATATTGGGTATTGTTCCT	GTGCTTGGGAGTCCTTGAAA	36.4	50.0	53.6	58.0	1154 bp	1.0	40.1
psbA_p2	CAAGATATTGGGTATTGTTCCT	AATACGTGTGCTTGGGAGTC	36.4	50.0	53.6	57.9	1161 bp	1.0	40.1
psbA_p3	TGGGTATTGTTCCTTAATTCA	GTGCTTGGGAGTCCTTGAAA	33.3	50.0	52.4	58.0	1146 bp	1.0	40.1
psbA_p4	CAAGATATTGGGTATTGTTCCTT	GTGCTTGGGAGTCCTTGAAA	34.8	50.0	54.4	58.0	1154 bp	1.0	40.1
psbA_p5	TGGGTATTGTTCCTTAATTCA	AATACGTGTGCTTGGGAGTC	33.3	50.0	52.4	57.9	1153 bp	1.0	40.1