Hotspot

ndhA

SSC · 9847-11991 · 2145 bp

Back to Werauhia

Pi 0.0028

MarkerSeek score 59.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2145
variable_sites: 6
pi: 0.0028
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGGACCCAATTACCATAGGGA	TGGGAGAAGTTGATCGTTGA	47.6	45.0	57.8	56.5	2279-2283 bp	1.0	58.9
ndhA_p2	TGGACCCAATTACCATAGGGA	TGGGAGAAGTTGATCGTTGAA	47.6	42.9	57.8	57.2	2279-2283 bp	1.0	56.9
ndhA_p3	TGGACCCAATTACCATAGGGA	ATGGGAGAAGTTGATCGTTGA	47.6	42.9	57.8	57.0	2280-2284 bp	1.0	55.2
ndhA_p4	TGGACCCAATTACCATAGGGA	CACCCGGTTTTATCAATTTGCA	47.6	40.9	57.8	58.1	2375-2379 bp	1.0	54.4
ndhA_p5	TGGACCCAATTACCATAGGG	TGGGAGAAGTTGATCGTTGA	50.0	45.0	56.3	56.5	2279-2283 bp	1.0	54.2