Hotspot

matK

LSC · 46738-48273 · 1536 bp

Back to Werauhia

Pi 0.00521

MarkerSeek score 61.70

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1536
variable_sites: 8
pi: 0.00521
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TGCTTCAACCACTACACCAC	CCAAGAAAAACCTCCTGCCT	50.0	50.0	58.0	58.0	1686 bp	1.0	80.1
matK_p2	GCTTCAACCACTACACCACT	CCAAGAAAAACCTCCTGCCT	50.0	50.0	57.7	58.0	1685 bp	1.0	78.3
matK_p3	TGCTTCAACCACTACACCAC	ACCAAGAAAAACCTCCTGCC	50.0	50.0	58.0	58.3	1687 bp	1.0	77.7
matK_p4	TGCTTCAACCACTACACCAC	GAAAAACCTCCTGCCTCTGG	50.0	55.0	58.0	58.5	1682 bp	1.0	76.4
matK_p5	GCTTCAACCACTACACCACT	ACCAAGAAAAACCTCCTGCC	50.0	50.0	57.7	58.3	1686 bp	1.0	76.0