Hotspot

psbE-petL

LSC · 110909-112406 · 1498 bp

Back to Werauhia

Pi 0.00402

MarkerSeek score 61.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1498
variable_sites: 6
pi: 0.00402
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.9	58.5	1638-1645 bp	1.0	76.4
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.9	58.5	1636-1643 bp	1.0	76.4
psbE-petL_p3	ACTGACGAATAACCAACCCG	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.9	58.5	1651-1658 bp	1.0	76.1
psbE-petL_p4	ACTGACGAATAACCAACCCG	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.9	58.5	1649-1656 bp	1.0	76.1
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AAGCAGCCAGTAGAAAACCG	45.0	50.0	58.2	58.5	1647-1654 bp	1.0	75.5