Hotspot

accD-psaI

LSC · 104665-105496 · 832 bp

Back to Werauhia

Pi 0.00481

MarkerSeek score 56.80

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 832
variable_sites: 4
pi: 0.00481
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGCTCCATGGTTTCTTTCCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	946-957 bp	1.0	78.4
accD-psaI_p2	GCTCCATGGTTTCTTTCCCT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	945-956 bp	1.0	78.4
accD-psaI_p3	AGCTCCATGGTTTCTTTCCC	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.8	57.9	952-963 bp	1.0	77.8
accD-psaI_p4	GCTCCATGGTTTCTTTCCCT	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.8	57.9	951-962 bp	1.0	77.8
accD-psaI_p5	GACCCAATCGTACCACGTAA	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.3	58.0	998-1009 bp	1.0	75.0