Hotspot

rbcL-accD

LSC · 102510-103197 · 688 bp

Back to Werauhia

Pi 0.00298

MarkerSeek score 62.70

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 688
variable_sites: 2
pi: 0.00298
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CTAGCAAATGGAGCCCTGAG	ATCACTTCCACTGGTATGGC	55.0	50.0	58.0	57.3	906-944 bp	1.0	75.5
rbcL-accD_p2	TCGAGTTCGAACCGGTAGAT	ATCACTTCCACTGGTATGGC	50.0	50.0	58.3	57.3	849-887 bp	1.0	73.9
rbcL-accD_p3	TGAAGCTAGCAAATGGAGCC	ATCACTTCCACTGGTATGGC	50.0	50.0	58.2	57.3	911-949 bp	1.0	73.8
rbcL-accD_p4	CCGTGAAGCTAGCAAATGGA	ATCACTTCCACTGGTATGGC	50.0	50.0	58.3	57.3	914-952 bp	1.0	73.7
rbcL-accD_p5	TCCGTGAAGCTAGCAAATGG	ATCACTTCCACTGGTATGGC	50.0	50.0	58.3	57.3	915-953 bp	1.0	73.7