Hotspot

rpoB-trnC-GCA

LSC · 71748-73080 · 1333 bp

Back to Werauhia

Pi 0.00451

MarkerSeek score 53.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1333
variable_sites: 6
pi: 0.00451
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCCCTCATTTCCATTCCGGA	GCGACACCCAGATTTGAACT	50.0	50.0	58.4	58.5	1426 bp	1.0	73.4
rpoB-trnC-GCA_p2	TTCCCTCATTTCCATTCCGG	GCGACACCCAGATTTGAACT	50.0	50.0	57.6	58.5	1427 bp	1.0	73.2
rpoB-trnC-GCA_p3	TCCCTCATTTCCATTCCGGA	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.4	57.5	1401 bp	1.0	73.0
rpoB-trnC-GCA_p4	TTCCCTCATTTCCATTCCGG	TAAAGGATTTGCAGTCCCCC	50.0	50.0	57.6	57.5	1402 bp	1.0	72.8
rpoB-trnC-GCA_p5	TCCCTCATTTCCATTCCGGA	TTTGTTGATCAGGCGACACC	50.0	50.0	58.4	58.8	1438 bp	1.0	71.1