Hotspot

atpH-atpI

LSC · 58525-59472 · 948 bp

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Pi 0.0054

MarkerSeek score 50.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 948
variable_sites: 5
pi: 0.0054
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CCATGGAGGGTCATCATTGA	45.0	50.0	58.0	57.0	996-1000 bp	1.0	73.6
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TCCATGGAGGGTCATCATTG	45.0	50.0	58.0	57.0	997-1001 bp	1.0	73.6
atpH-atpI_p3	CAGTACCTTGACCAACTCCG	CCATGGAGGGTCATCATTGA	55.0	50.0	57.9	57.0	1055-1059 bp	1.0	73.1
atpH-atpI_p4	CAGTACCTTGACCAACTCCG	TCCATGGAGGGTCATCATTG	55.0	50.0	57.9	57.0	1056-1060 bp	1.0	73.1
atpH-atpI_p5	AGCCAATCCAGCAGCAATAA	CCATGGAGGGTCATCATTGA	45.0	50.0	57.9	57.0	1012-1016 bp	1.0	72.7