Hotspot

trnS-GCU-trnG-UCC

LSC · 52616-53764 · 1149 bp

Back to Werauhia

Pi 0.00177

MarkerSeek score 61.10

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1149
variable_sites: 2
pi: 0.00177
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	GAGAAAGCGGAAAGAGAGGG	AACCGATGGACCCCTTAACT	55.0	50.0	58.0	58.3	1316-1325 bp	1.0	78.3
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	AACCGATGGACCCCTTAACT	45.0	50.0	58.0	58.3	1261-1270 bp	1.0	78.2
trnS-GCU-trnG-UCC_p3	GAGAAAGCGGAAAGAGAGGG	AAACCGATGGACCCCTTAAC	55.0	50.0	58.0	57.5	1317-1326 bp	1.0	76.9
trnS-GCU-trnG-UCC_p4	ATTAGCAATCCGCCGCTTTA	AAACCGATGGACCCCTTAAC	45.0	50.0	58.0	57.5	1262-1271 bp	1.0	76.8
trnS-GCU-trnG-UCC_p5	GAGAAAGCGGAAAGAGAGGG	ACCGATGGACCCCTTAACTA	55.0	50.0	58.0	57.4	1315-1324 bp	1.0	76.5