Hotspot

psbA-trnK-UUU

LSC · 46218-46444 · 227 bp

Back to Werauhia

Pi 0.0132

MarkerSeek score 51.80

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 227
variable_sites: 3
pi: 0.0132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TAGGCTTGTACTTTCGCGTC	TGGGGAGGGATCTTTTTCCT	50.0	50.0	58.0	57.9	353 bp	1.0	80.0
psbA-trnK-UUU_p2	AACACCGAACCATCCAATGT	TGGGGAGGGATCTTTTTCCT	45.0	50.0	57.7	57.9	419 bp	1.0	77.7
psbA-trnK-UUU_p3	GCTTGTACTTTCGCGTCTCT	TGGGGAGGGATCTTTTTCCT	50.0	50.0	58.3	57.9	350 bp	1.0	77.7
psbA-trnK-UUU_p4	TCAAAACACCGAACCATCCA	TGGGGAGGGATCTTTTTCCT	45.0	50.0	57.6	57.9	423 bp	1.0	77.2
psbA-trnK-UUU_p5	TAGGCTTGTACTTTCGCGTC	ACTCCATCCGACTAGTTCCG	50.0	55.0	58.0	58.6	308 bp	1.0	75.8