Hotspot

rps19-psbA

LSC · 44994-45155 · 162 bp

Back to Werauhia

Pi 0.0186

MarkerSeek score 63.80

Primer available yes

Label rps19-psbA

Feature Metadata

label_name: rps19-psbA
feature_type: igs
region: LSC
length_bp: 162
variable_sites: 3
pi: 0.0186
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-psbA_p1	TCTGACTTTCGCGAGACATG	AGCTGTTGTTGAAGCTCCAT	50.0	45.0	58.0	57.7	244-245 bp	1.0	78.9
rps19-psbA_p2	TCTGACTTTCGCGAGACATG	ATGCTCACAACTTCCCCCTA	50.0	50.0	58.0	58.3	269-270 bp	1.0	78.4
rps19-psbA_p3	CCTACTCTGACTTTCGCGAG	AGCTGTTGTTGAAGCTCCAT	55.0	45.0	57.8	57.7	249-250 bp	1.0	77.6
rps19-psbA_p4	CCTACTCTGACTTTCGCGAG	ATGCTCACAACTTCCCCCTA	55.0	50.0	57.8	58.3	274-275 bp	1.0	77.1
rps19-psbA_p5	TCTGACTTTCGCGAGACATG	TAATGCTCACAACTTCCCCC	50.0	50.0	58.0	57.5	271-272 bp	1.0	77.0