Hotspot

ycf1

SSC · 128233-133502 · 5270 bp

Back to Weigela

Pi 0.0122

MarkerSeek score 45.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5270
variable_sites: 96
pi: 0.0122
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCCTCCTTCTTCTGTTTCCG	AGGATTCGAACCTACGACCA	55.0	50.0	57.9	58.2	1208-1406 bp	1.0	86.6
ycf1_p2	CCCTCCTTCTTCTGTTTCCG	GGATTCGAACCTACGACCAG	55.0	55.0	57.9	57.8	1207-1405 bp	1.0	86.2
ycf1_p3	CCCTCCTTCTTCTGTTTCCG	GGTCAAAGCGAGCCTTTTTG	55.0	50.0	57.9	58.2	1309-1507 bp	1.0	85.7
ycf1_p4	CCCTCCTTCTTCTGTTTCCG	ACCACTGAGCTACTGAGGAA	55.0	50.0	57.9	57.7	1165-1363 bp	1.0	85.6
ycf1_p5	CCCTCCTTCTTCTGTTTCCG	CCCCAAGTAGGATTCGAACC	55.0	55.0	57.9	57.7	1216-1414 bp	1.0	85.2