Hotspot

accD

LSC · 60034-61820 · 1787 bp

Back to Weigela

Pi 0.0101

MarkerSeek score 56.30

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1787
variable_sites: 24
pi: 0.0101
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	GCTCGAATTCAAATGAGCGG	TAAGGCGGGAGGAGGAAATT	50.0	50.0	57.9	58.4	2018-2284 bp	1.0	81.0
accD_p2	CCCCTCTGCTCGAATTCAAA	TAAGGCGGGAGGAGGAAATT	50.0	50.0	57.9	58.4	2025-2291 bp	1.0	80.8
accD_p3	TCAAATGAGCGGGAAGAAGG	TAAGGCGGGAGGAGGAAATT	50.0	50.0	57.9	58.4	2010-2276 bp	1.0	80.8
accD_p4	GATTACAACGAGGCCCAGAG	TAAGGCGGGAGGAGGAAATT	55.0	50.0	58.1	58.4	2275 bp	0.333333	49.6
accD_p5	GATTACAACGAGGCCCAGAG	GCGGGAGGAGGAAATTTGTT	55.0	50.0	58.1	58.5	2271 bp	0.333333	49.2