Hotspot

ycf2-trnI-CAU

IRa · 156511-156786 · 276 bp

Back to Weigela

Pi 0.017

MarkerSeek score 63.60

Primer available yes

Label ycf2-trnI-CAU

Feature Metadata

label_name: ycf2-trnI-CAU
feature_type: igs
region: IRa
length_bp: 276
variable_sites: 7
pi: 0.017
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnI-CAU_p1	AGGGTTGTAGTCTTCTCATTGTG	GTTGGGCGCTTTAACCATTC	43.5	50.0	58.1	58.0	331-332 bp	1.0	57.1
ycf2-trnI-CAU_p2	AGGGTTGTAGTCTTCTCATTGTG	ATTGGCATGCATCCAGTAGG	43.5	50.0	58.1	58.0	380-381 bp	1.0	56.8
ycf2-trnI-CAU_p3	CTGAATTGAATTCGGTCCATGA	GTTGGGCGCTTTAACCATTC	40.9	50.0	56.8	58.0	412-413 bp	1.0	55.6
ycf2-trnI-CAU_p4	AGGGTTGTAGTCTTCTCATTGT	ATTGGCATGCATCCAGTAGG	40.9	50.0	56.7	58.0	380-381 bp	1.0	55.6
ycf2-trnI-CAU_p5	CTGAATTGAATTCGGTCCATGA	ATTGGCATGCATCCAGTAGG	40.9	50.0	56.8	58.0	461-462 bp	1.0	55.5