Hotspot

rpoB-trnC-GCA

LSC · 28257-29531 · 1275 bp

Back to Weigela

Pi 0.00261

MarkerSeek score 61.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1275
variable_sites: 5
pi: 0.00261
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCCCTCATTTCCATCCTGGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.7	58.1	1349-1373 bp	1.0	78.2
rpoB-trnC-GCA_p2	GTTCTTCCATCAAGCCCTGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1428-1452 bp	1.0	76.4
rpoB-trnC-GCA_p3	AGTTCTTCCATCAAGCCCTG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1429-1453 bp	1.0	76.4
rpoB-trnC-GCA_p4	TCCCTCATTTCCATCCTGGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.7	58.4	1331-1355 bp	1.0	76.0
rpoB-trnC-GCA_p5	CTTCCATCAAGCCCTGATCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.3	58.1	1425-1449 bp	1.0	74.6