Hotspot

trnK-UUU-rps16

LSC · 4326-5194 · 869 bp

Back to Weigela

Pi 0.00155
MarkerSeek score 60.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
869
variable_sites
2
pi
0.00155
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC TTGAAAAAGGTGCTCAGCCT 55.0 45.0 57.9 57.9 1055-1065 bp 1.0 79.7
trnK-UUU-rps16_p2 CGATGGTATGGACGAATCCC TTGAAAAAGGTGCTCAGCCT 55.0 45.0 57.9 57.9 1054-1064 bp 1.0 79.7
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT TTGAAAAAGGTGCTCAGCCT 50.0 45.0 58.2 57.9 994-1004 bp 1.0 79.0
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TTGAAAAAGGTGCTCAGCCT 50.0 45.0 58.2 57.9 993-1003 bp 1.0 79.0
trnK-UUU-rps16_p5 CGATGGTATGGACGAATCCC AAAAGGTGCTCAGCCTACAG 55.0 50.0 57.9 57.8 1050-1060 bp 1.0 78.7