primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	CAATCCACTGCCTTGATCCA	GAGCTTAGTTTCCGTCTGGG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.144821	69	412	1046-1049	1.000000	81.1
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	GGAGCTTAGTTTCCGTCTGG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.144821	69	412	1047-1050	1.000000	81.1
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	GGGAGCTTAGTTTCCGTCTG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.144821	69	412	1048-1051	1.000000	81.1
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	CAATCCACTGCCTTGATCCA	GAGTGGGAGCTTAGTTTCCG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.144821	69	412	1052-1055	1.000000	81.0
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	GAGCTTAGTTTCCGTCTGGG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.146329	69	412	1047-1050	1.000000	81.0
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCGATGGTATGGACGAATCC	TTGAAAAAGGTGCTCAGCCT	20	20	55.0	45.0	57.9	57.9	0.0	0.0	0.152064	4326	5194	1055-1065	1.000000	79.7
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CGATGGTATGGACGAATCCC	TTGAAAAAGGTGCTCAGCCT	20	20	55.0	45.0	57.9	57.9	0.0	0.0	0.152064	4326	5194	1054-1064	1.000000	79.7
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAAGCCGAGTACTCTACCGT	TTGAAAAAGGTGCTCAGCCT	20	20	50.0	45.0	58.2	57.9	20.3	0.0	0.240197	4326	5194	994-1004	1.000000	79.0
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	TTGAAAAAGGTGCTCAGCCT	20	20	50.0	45.0	58.2	57.9	20.3	0.0	0.240197	4326	5194	993-1003	1.000000	79.0
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CGATGGTATGGACGAATCCC	AAAAGGTGCTCAGCCTACAG	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.271414	4326	5194	1050-1060	1.000000	78.7
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	TCCCTCATTTCCATCCTGGA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.7	58.1	0.0	0.0	0.367403	28257	29531	1349-1373	1.000000	78.2
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	GTTCTTCCATCAAGCCCTGA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.5	58.1	0.0	0.0	0.579026	28257	29531	1428-1452	1.000000	76.4
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	AGTTCTTCCATCAAGCCCTG	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.5	58.1	0.0	0.0	0.586023	28257	29531	1429-1453	1.000000	76.4
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TCCCTCATTTCCATCCTGGA	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	57.7	58.4	0.0	14.6	0.640766	28257	29531	1331-1355	1.000000	76.0
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	CTTCCATCAAGCCCTGATCA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.3	58.1	0.0	0.0	0.805450	28257	29531	1425-1449	1.000000	74.6
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	CAAAGAGGCCCCTTTAGCTC	GGACCAGCCTACAAAAACGA	20	20	55.0	50.0	58.2	58.1	12.5	0.0	0.356683	34263	35111	1036-1039	1.000000	77.5
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	GGCGTAAGTCATCGGTTCAA	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.395920	34263	35111	992-995	1.000000	77.2
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	CAAAGAGGCCCCTTTAGCTC	TCTCCGTAACCAGTCATCCA	20	20	55.0	50.0	58.2	57.8	12.5	0.0	0.468952	34263	35111	1009-1012	1.000000	76.6
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	AATCCGATAAGGGGCTTTGG	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	57.6	58.1	0.0	0.0	0.478086	34263	35111	973-976	1.000000	76.6
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GGCGTAAGTCATCGGTTCAA	TCTCCGTAACCAGTCATCCA	20	20	50.0	50.0	58.3	57.8	0.0	0.0	0.508188	34263	35111	965-968	1.000000	76.3
accD_p1	accD	1	GCTCGAATTCAAATGAGCGG	TAAGGCGGGAGGAGGAAATT	20	20	50.0	50.0	57.9	58.4	14.6	0.0	0.505031	60034	61820	2018-2284	1.000000	81.0
accD_p2	accD	2	CCCCTCTGCTCGAATTCAAA	TAAGGCGGGAGGAGGAAATT	20	20	50.0	50.0	57.9	58.4	6.2	0.0	0.522092	60034	61820	2025-2291	1.000000	80.8
accD_p3	accD	3	TCAAATGAGCGGGAAGAAGG	TAAGGCGGGAGGAGGAAATT	20	20	50.0	50.0	57.9	58.4	0.0	0.0	0.522092	60034	61820	2010-2276	1.000000	80.8
accD_p4	accD	4	GATTACAACGAGGCCCAGAG	TAAGGCGGGAGGAGGAAATT	20	20	55.0	50.0	58.1	58.4	0.0	0.0	0.466907	60034	61820	2275-2275	0.333333	49.6
accD_p5	accD	5	GATTACAACGAGGCCCAGAG	GCGGGAGGAGGAAATTTGTT	20	20	55.0	50.0	58.1	58.5	0.0	0.0	0.512834	60034	61820	2271-2271	0.333333	49.2
ycf4-cemA_p1	ycf4-cemA	1	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	58.1	58.1	3.3	0.0	0.172357	63636	64450	919-927	1.000000	79.7
ycf4-cemA_p2	ycf4-cemA	2	ACAAAAAGCTGCCGAATTGG	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.230011	63636	64450	935-943	1.000000	79.2
ycf4-cemA_p3	ycf4-cemA	3	TTGGCCTATTTCTTGCGTGT	CAGGGCAAAAAGACGAGAGA	20	20	45.0	50.0	58.1	57.8	3.3	0.0	0.265546	63636	64450	908-916	1.000000	78.9
ycf4-cemA_p4	ycf4-cemA	4	ACAAAAAGCTGCCGAATTGG	CAGGGCAAAAAGACGAGAGA	20	20	45.0	50.0	57.8	57.8	0.0	0.0	0.323199	63636	64450	924-932	1.000000	78.5
ycf4-cemA_p5	ycf4-cemA	5	TTGGCCTATTTCTTGCGTGT	ATCCACCAGGGCAAAAAGAC	20	20	45.0	50.0	58.1	58.4	3.3	0.0	0.463125	63636	64450	914-922	1.000000	77.3
petA-psbJ_p1	petA-psbJ	1	GGCATCTGTGATTTTGGCAC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	58.0	57.6	0.0	4.1	0.365402	66334	67387	1120-1165	1.000000	80.5
petA-psbJ_p2	petA-psbJ	2	ATCCATTACGTGTCCAAGGC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	58.0	57.6	0.0	4.1	0.403359	66334	67387	1154-1199	1.000000	80.1
petA-psbJ_p3	petA-psbJ	3	GGCCTTTTGTTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.8	57.6	0.0	4.1	0.519826	66334	67387	1137-1182	1.000000	79.2
petA-psbJ_p4	petA-psbJ	4	CATTACGTGTCCAAGGCCTT	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	58.2	57.6	13.8	4.1	0.543756	66334	67387	1151-1196	1.000000	79.0
petA-psbJ_p5	petA-psbJ	5	TTACGTGTCCAAGGCCTTTT	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	57.6	57.6	17.6	4.1	0.716678	66334	67387	1149-1194	1.000000	77.6
rps18_p1	rps18	1	CGACCGAAATAGGATTTTTGGG	CGAGCAAAACCTCAATCTGC	22	20	45.5	50.0	57.7	57.7	7.8	0.0	2.540630	71729	72202	565-628	1.000000	63.3
rps18_p2	rps18	2	CGACCGAAATAGGATTTTTGGG	GAGCAAAACCTCAATCTGCG	22	20	45.5	50.0	57.7	57.7	7.8	0.0	2.540630	71729	72202	564-627	1.000000	63.3
rps18_p3	rps18	3	CGACCGAAATAGGATTTTTGGG	AGCAAAACCTCAATCTGCGT	22	20	45.5	45.0	57.7	58.4	7.8	0.0	2.678030	71729	72202	563-626	1.000000	62.2
rps18_p4	rps18	4	CGACCGAAATAGGATTTTTGGG	GCAAAACCTCAATCTGCGTT	22	20	45.5	45.0	57.7	57.6	7.8	0.0	2.709514	71729	72202	562-625	1.000000	62.0
rps18_p5	rps18	5	CGACCGAAATAGGATTTTTGGG	ACCTCAATCTGCGTTTGAGT	22	20	45.5	45.0	57.7	57.4	7.8	18.9	2.838068	71729	72202	557-620	1.000000	61.0
rpoA_p1	rpoA	1	AAACCCATTTTCCGCTTCCT	CAGATGTACGGGGTCAAGTG	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.019826	81885	82880	1412-1430	1.000000	81.1
rpoA_p2	rpoA	2	AACCCATTTTCCGCTTCCTT	CAGATGTACGGGGTCAAGTG	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.019826	81885	82880	1411-1429	1.000000	81.1
rpoA_p3	rpoA	3	ACCCATTTTCCGCTTCCTTT	CAGATGTACGGGGTCAAGTG	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.019826	81885	82880	1410-1428	1.000000	81.1
rpoA_p4	rpoA	4	CCGCGGGATTGTTAACAGTA	CAGATGTACGGGGTCAAGTG	20	20	50.0	55.0	58.0	58.0	20.1	0.0	0.023082	81885	82880	1732-1750	1.000000	80.8
rpoA_p5	rpoA	5	TAATGGTTTCAGTACCCGCG	CAGATGTACGGGGTCAAGTG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.023082	81885	82880	1747-1765	1.000000	80.8
rpl22_p1	rpl22	1	GTTGTGCAAACCAAAGGGAA	TCGCGTCGTTAAGAAATGTT	20	20	45.0	40.0	57.3	56.1	0.0	5.8	2.557779	87467	87958	635-671	1.000000	63.1
rpl22_p2	rpl22	2	GTTGTGCAAACCAAAGGGAA	GATCGCGTCGTTAAGAAATGT	20	21	45.0	42.9	57.3	57.1	0.0	5.8	2.583823	87467	87958	637-673	1.000000	62.9
rpl22_p3	rpl22	3	TGTGCAAACCAAAGGGAATG	TCGCGTCGTTAAGAAATGTT	20	20	45.0	40.0	57.1	56.1	0.0	5.8	2.782315	87467	87958	633-669	1.000000	61.3
rpl22_p4	rpl22	4	GTTGTGCAAACCAAAGGGAA	ATCGCGTCGTTAAGAAATGT	20	20	45.0	40.0	57.3	55.9	0.0	5.8	2.783684	87467	87958	636-672	1.000000	61.3
rpl22_p5	rpl22	5	TGTGCAAACCAAAGGGAATG	GATCGCGTCGTTAAGAAATGT	20	21	45.0	42.9	57.1	57.1	0.0	5.8	2.808359	87467	87958	635-671	1.000000	61.1
trnI-CAU-ycf2_p1	trnI-CAU-ycf2	1	GTTGGGCGCTTTAACCATTC	AGGGTTGTAGTCTTCTCATTGTG	20	23	50.0	43.5	58.0	58.1	7.6	0.0	3.109699	90419	90695	331-332	1.000000	57.1
trnI-CAU-ycf2_p2	trnI-CAU-ycf2	2	ATTGGCATGCATCCAGTAGG	AGGGTTGTAGTCTTCTCATTGTG	20	23	50.0	43.5	58.0	58.1	21.4	0.0	3.109377	90419	90695	380-381	1.000000	56.8
trnI-CAU-ycf2_p3	trnI-CAU-ycf2	3	GTTGGGCGCTTTAACCATTC	CTGAATTGAATTCGGTCCATGA	20	22	50.0	40.9	58.0	56.8	7.6	16.6	3.242004	90419	90695	412-413	1.000000	55.6
trnI-CAU-ycf2_p4	trnI-CAU-ycf2	4	ATTGGCATGCATCCAGTAGG	AGGGTTGTAGTCTTCTCATTGT	20	22	50.0	40.9	58.0	56.7	21.4	0.0	3.261853	90419	90695	380-381	1.000000	55.6
trnI-CAU-ycf2_p5	trnI-CAU-ycf2	5	ATTGGCATGCATCCAGTAGG	CTGAATTGAATTCGGTCCATGA	20	22	50.0	40.9	58.0	56.8	21.4	16.6	3.241683	90419	90695	461-462	1.000000	55.5
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	AGGATTCGAACCTACGACCA	CTGGAAGTGGAAGGAAAGGT	20	20	50.0	50.0	58.2	57.0	15.0	0.0	1.116097	113220	115386	2136-2565	1.000000	80.0
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	GGATTCGAACCTACGACCAG	CTGGAAGTGGAAGGAAAGGT	20	20	55.0	50.0	57.8	57.0	0.0	0.0	1.164772	113220	115386	2135-2564	1.000000	79.6
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	CCCCAAGTAGGATTCGAACC	CTGGAAGTGGAAGGAAAGGT	20	20	55.0	50.0	57.7	57.0	0.0	0.0	1.286665	113220	115386	2144-2573	1.000000	78.6
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	TCTCCCCAAGTAGGATTCGA	CTGGAAGTGGAAGGAAAGGT	20	20	50.0	50.0	57.2	57.0	0.0	0.0	1.800105	113220	115386	2147-2576	1.000000	74.5
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	AGGATTCGAACCTACGACCA	TGTTGCTGTCGGAAAAAGAAG	20	21	50.0	42.9	58.2	57.3	15.0	0.0	1.847681	113220	115386	2183-2612	1.000000	74.0
ycf1_p1	ycf1	1	CCCTCCTTCTTCTGTTTCCG	AGGATTCGAACCTACGACCA	20	20	55.0	50.0	57.9	58.2	0.0	15.0	0.257236	133383	133502	1208-1406	1.000000	86.6
ycf1_p2	ycf1	2	CCCTCCTTCTTCTGTTTCCG	GGATTCGAACCTACGACCAG	20	20	55.0	55.0	57.9	57.8	0.0	0.0	0.305911	133383	133502	1207-1405	1.000000	86.2
ycf1_p3	ycf1	3	CCCTCCTTCTTCTGTTTCCG	GGTCAAAGCGAGCCTTTTTG	20	20	55.0	50.0	57.9	58.2	0.0	0.0	0.329648	133383	133502	1309-1507	1.000000	85.7
ycf1_p4	ycf1	4	CCCTCCTTCTTCTGTTTCCG	ACCACTGAGCTACTGAGGAA	20	20	55.0	50.0	57.9	57.7	0.0	11.2	0.411384	133383	133502	1165-1363	1.000000	85.6
ycf1_p5	ycf1	5	CCCTCCTTCTTCTGTTTCCG	CCCCAAGTAGGATTCGAACC	20	20	55.0	55.0	57.9	57.7	0.0	0.0	0.427805	133383	133502	1216-1414	1.000000	85.2
ycf1-trnN-GUU_p1	ycf1-trnN-GUU	1	GGGCCCTATGAAGAATGTGG	GGCAGAATAGCAGAGCAAGT	20	20	55.0	50.0	58.0	58.0	17.0	0.0	0.048648	133503	134042	905-1103	1.000000	89.2
ycf1-trnN-GUU_p2	ycf1-trnN-GUU	2	GGGCCCTATGAAGAATGTGG	GAACTTGGGTGTGGGTCTTT	20	20	55.0	50.0	58.0	57.9	17.0	0.0	0.080483	133503	134042	1019-1217	1.000000	88.5
ycf1-trnN-GUU_p3	ycf1-trnN-GUU	3	GGGCCCTATGAAGAATGTGG	GGGTGTGGGTCTTTTGTCTT	20	20	55.0	50.0	58.0	57.9	17.0	0.0	0.080483	133503	134042	1013-1211	1.000000	88.5
ycf1-trnN-GUU_p4	ycf1-trnN-GUU	4	GGGCCCTATGAAGAATGTGG	CACAACCTTCCCAAAAGGGA	20	20	55.0	50.0	58.0	57.9	17.0	8.6	0.093772	133503	134042	1098-1296	1.000000	88.1
ycf1-trnN-GUU_p5	ycf1-trnN-GUU	5	GGGCCCTATGAAGAATGTGG	ATCATGATCGGGATAGCGGA	20	20	55.0	50.0	58.0	58.1	17.0	1.2	0.160485	133503	134042	1051-1249	1.000000	87.8
ycf2-trnI-CAU_p1	ycf2-trnI-CAU	1	AGGGTTGTAGTCTTCTCATTGTG	GTTGGGCGCTTTAACCATTC	23	20	43.5	50.0	58.1	58.0	0.0	7.6	3.109699	156511	156786	331-332	1.000000	57.1
ycf2-trnI-CAU_p2	ycf2-trnI-CAU	2	AGGGTTGTAGTCTTCTCATTGTG	ATTGGCATGCATCCAGTAGG	23	20	43.5	50.0	58.1	58.0	0.0	21.4	3.109377	156511	156786	380-381	1.000000	56.8
ycf2-trnI-CAU_p3	ycf2-trnI-CAU	3	CTGAATTGAATTCGGTCCATGA	GTTGGGCGCTTTAACCATTC	22	20	40.9	50.0	56.8	58.0	16.6	7.6	3.242004	156511	156786	412-413	1.000000	55.6
ycf2-trnI-CAU_p4	ycf2-trnI-CAU	4	AGGGTTGTAGTCTTCTCATTGT	ATTGGCATGCATCCAGTAGG	22	20	40.9	50.0	56.7	58.0	0.0	21.4	3.261853	156511	156786	380-381	1.000000	55.6
ycf2-trnI-CAU_p5	ycf2-trnI-CAU	5	CTGAATTGAATTCGGTCCATGA	ATTGGCATGCATCCAGTAGG	22	20	40.9	50.0	56.8	58.0	16.6	21.4	3.241683	156511	156786	461-462	1.000000	55.5