Hotspot

rps19

IRb · 85761-86039 · 279 bp

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Pi 0
MarkerSeek score 24.10
Primer available yes
Label rps19

Feature Metadata

label_name
rps19
feature_type
gene
region
IRb
length_bp
279
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
24.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19_p1 GCGAACCAAAAGGAACGATG GGGTAGAGCCGGATCTAAGT 50.0 55.0 58.0 58.0 1408 bp 1.0 79.9
rps19_p2 GCGAACCAAAAGGAACGATG TGGGTAGAGCCGGATCTAAG 50.0 55.0 58.0 58.0 1409 bp 1.0 79.9
rps19_p3 GCGAACCAAAAGGAACGATG GTTATCCTGCGCTTGGAAGA 50.0 50.0 58.0 58.0 1271 bp 1.0 79.7
rps19_p4 TGCTGCTGCAGAATAAACCA GGGTAGAGCCGGATCTAAGT 45.0 55.0 58.1 58.0 1093 bp 1.0 79.5
rps19_p5 GCGAACCAAAAGGAACGATG GAGGGTAAATCGGCCACATT 50.0 50.0 58.0 57.9 1521 bp 1.0 79.4