Hotspot

rps16

LSC · 4849-5993 · 1145 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1145
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GCAAGAAAAAGGCAAATTGTGT	CCGGTTGAGCCAATGACTAT	36.4	50.0	57.1	57.7	1320 bp	1.0	54.3
rps16_p2	GCAAGAAAAAGGCAAATTGTGT	TATACACACACCGGTTGAGC	36.4	50.0	57.1	57.6	1330 bp	1.0	54.0
rps16_p3	ACAGCAAGAAAAAGGCAAATTG	CCGGTTGAGCCAATGACTAT	36.4	50.0	56.9	57.7	1323 bp	1.0	52.2
rps16_p4	AGCAAGAAAAAGGCAAATTGTG	CCGGTTGAGCCAATGACTAT	36.4	50.0	56.9	57.7	1321 bp	1.0	52.2
rps16_p5	CAGCAAGAAAAAGGCAAATTGT	CCGGTTGAGCCAATGACTAT	36.4	50.0	56.9	57.7	1322 bp	1.0	52.2