Hotspot

rpl16

LSC · 82800-84345 · 1546 bp

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Pi 0.000651

MarkerSeek score 59.70

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1546
variable_sites: 1
pi: 0.000651
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GTCCAGAATAAACCTGCGGT	AGGTTGAATAAAAATTCGATTGACCA	50.0	30.8	57.9	58.1	1639-1650 bp	1.0	40.6
rpl16_p2	GTCCAGAATAAACCTGCGGT	GGTTGAATAAAAATTCGATTGACCA	50.0	32.0	57.9	56.9	1638-1649 bp	1.0	40.6
rpl16_p3	GTCCAGAATAAACCTGCGGT	AGGTTGAATAAAAATTCGATTGACC	50.0	32.0	57.9	56.6	1639-1650 bp	1.0	40.6
rpl16_p4	TCCGTCCAGAATAAACCTGC	AGGTTGAATAAAAATTCGATTGACCA	50.0	30.8	57.6	58.1	1642-1653 bp	1.0	40.6
rpl16_p5	TCCGTCCAGAATAAACCTGC	GGTTGAATAAAAATTCGATTGACCA	50.0	32.0	57.6	56.9	1641-1652 bp	1.0	40.6