Hotspot

rps19

IRb · 44385-44663 · 279 bp

Back to Wallisia

Pi 0

MarkerSeek score 23.70

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: IRb
length_bp: 279
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 23.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TCTGACCTCCATACTTAGATCGA	TCTATTCTTAATTCCAAATTCCAA	43.5	25.0	57.7	52.4	388 bp	1.0	40.0
rps19_p2	TCTGACCTCCATACTTAGATCGA	TTCTATTCTTAATTCCAAATTCCA	43.5	25.0	57.7	52.4	389 bp	1.0	40.0
rps19_p3	TGACCTCCATACTTAGATCGAGA	TCTATTCTTAATTCCAAATTCCAA	43.5	25.0	57.7	52.4	386 bp	1.0	40.0
rps19_p4	TGACCTCCATACTTAGATCGAGA	TTCTATTCTTAATTCCAAATTCCA	43.5	25.0	57.7	52.4	387 bp	1.0	40.0
rps19_p5	CTGACCTCCATACTTAGATCGAG	TCTATTCTTAATTCCAAATTCCAA	47.8	25.0	57.4	52.4	387 bp	1.0	40.0