Hotspot

rps15

SSC · 12857-13129 · 273 bp

Back to Wallisia

Pi 0
MarkerSeek score 30.80
Primer available yes
Label rps15

Feature Metadata

label_name
rps15
feature_type
gene
region
SSC
length_bp
273
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
30.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15_p1 TCTTGTAACCGGTACTGTCA TCCTGATCGGAAAAATCCATGA 45.0 40.9 55.8 57.5 446 bp 1.0 42.7
rps15_p2 TCCTCAAAACGAGACTCATCA TCCTGATCGGAAAAATCCATGA 42.9 40.9 56.7 57.5 392 bp 1.0 41.5
rps15_p3 TTCTTGTAACCGGTACTGTCA TCCTGATCGGAAAAATCCATGA 42.9 40.9 56.6 57.5 447 bp 1.0 41.0
rps15_p4 TCTTGTAACCGGTACTGTCAT TCCTGATCGGAAAAATCCATGA 42.9 40.9 56.4 57.5 446 bp 1.0 40.0
rps15_p5 TCCTCAAAACGAGACTCATCAA TCCTGATCGGAAAAATCCATGA 40.9 40.9 57.4 57.5 392 bp 1.0 40.0