Hotspot

ndhI

SSC · 8800-9342 · 543 bp

Back to Wallisia

Pi 0.00184

MarkerSeek score 58.60

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 543
variable_sites: 1
pi: 0.00184
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	CCCAAAATAAAAAGCTTGGATCCT	ACAACTTCTTCCCAACTTGT	37.5	40.0	57.9	55.0	703 bp	1.0	40.1
ndhI_p2	ACCCAAAATAAAAAGCTTGGATCC	ACAACTTCTTCCCAACTTGT	37.5	40.0	58.2	55.0	704 bp	1.0	40.1
ndhI_p3	CCCAAAATAAAAAGCTTGGATCCT	AACAACTTCTTCCCAACTTGT	37.5	38.1	57.9	55.8	704 bp	1.0	40.1
ndhI_p4	CCCAAAATAAAAAGCTTGGATCCT	ACAACTTCTTCCCAACTTGTT	37.5	38.1	57.9	55.8	703 bp	1.0	40.1
ndhI_p5	ACCCAAAATAAAAAGCTTGGATCC	ACAACTTCTTCCCAACTTGTT	37.5	38.1	58.2	55.8	704 bp	1.0	40.1