Hotspot

ndhA

SSC · 9431-11575 · 2145 bp

Back to Wallisia

Pi 0.000466

MarkerSeek score 57.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2145
variable_sites: 1
pi: 0.000466
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGGACCCAATTACCATAGGGA	TGGGAGAAGTTGATCGTTGA	47.6	45.0	57.8	56.5	2279 bp	1.0	58.7
ndhA_p2	TGGACCCAATTACCATAGGGA	TGGGAGAAGTTGATCGTTGAA	47.6	42.9	57.8	57.2	2279 bp	1.0	56.7
ndhA_p3	TGGACCCAATTACCATAGGGA	ATGGGAGAAGTTGATCGTTGA	47.6	42.9	57.8	57.0	2280 bp	1.0	55.0
ndhA_p4	TGGACCCAATTACCATAGGGA	CACCCGGTTTTATCAATTTGCA	47.6	40.9	57.8	58.1	2375 bp	1.0	54.2
ndhA_p5	TGGACCCAATTACCATAGGG	TGGGAGAAGTTGATCGTTGA	50.0	45.0	56.3	56.5	2279 bp	1.0	54.0