Hotspot

atpF

LSC · 56106-57492 · 1387 bp

Back to Wallisia

Pi 0.00288

MarkerSeek score 60.40

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1387
variable_sites: 4
pi: 0.00288
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACTAATTTCGTCGGCTCGAA	GGGCGAGCGAAGTAATAGAA	45.0	50.0	57.4	57.5	1561 bp	1.0	71.0
atpF_p2	GGCTCGAAGGGTTACCATTA	GGGCGAGCGAAGTAATAGAA	50.0	50.0	57.0	57.5	1549 bp	1.0	68.2
atpF_p3	ACTAATTTCGTCGGCTCGAA	AAGGGGCGAGCGAAGTAATA	45.0	50.0	57.4	58.9	1564 bp	1.0	68.0
atpF_p4	TCTTTTTCGGAAGCAAAGGGA	GGGCGAGCGAAGTAATAGAA	42.9	50.0	58.1	57.5	1517 bp	1.0	67.7
atpF_p5	GGCTCGAAGGGTTACCATTAG	GGGCGAGCGAAGTAATAGAA	52.4	50.0	58.2	57.5	1549 bp	1.0	66.9