Hotspot

accD

LSC · 103050-104510 · 1461 bp

Back to Wallisia

Pi 0.000684

MarkerSeek score 59.10

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1461
variable_sites: 1
pi: 0.000684
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TCAAATAGGGGGCAGGAAGA	ACTTGTTCGCTACAAACAAA	50.0	35.0	58.0	54.0	1528 bp	1.0	47.8
accD_p2	TCAAATAGGGGGCAGGAAGA	CAAACAGGGATTCAAAGTGT	50.0	40.0	58.0	54.0	1605-1606 bp	1.0	47.8
accD_p3	TCATCAAATAGGGGGCAGGA	ACTTGTTCGCTACAAACAAA	50.0	35.0	58.1	54.0	1531 bp	1.0	47.2
accD_p4	TCATCAAATAGGGGGCAGGA	CAAACAGGGATTCAAAGTGT	50.0	40.0	58.1	54.0	1608-1609 bp	1.0	47.2
accD_p5	TCAAATAGGGGGCAGGAAGA	ACTTGTTCGCTACAAACAAAA	50.0	33.3	58.0	54.8	1528 bp	1.0	46.6