Hotspot

rpl20-rps12

LSC · 115409-116112 · 704 bp

Back to Wallisia

Pi 0

MarkerSeek score 58.90

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 704
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	AGTCTTGAATGAGCCCCTCT	TCTCCCGCTCTTAGAGGATG	50.0	55.0	58.0	58.0	849-850 bp	1.0	79.7
rpl20-rps12_p2	TGAGCCCCTCTAAAGGTTGA	TCTCCCGCTCTTAGAGGATG	50.0	55.0	58.0	58.0	840-841 bp	1.0	79.7
rpl20-rps12_p3	TTTTTGTTCGACGTCTCCGA	TCTCCCGCTCTTAGAGGATG	45.0	55.0	57.8	58.0	806-807 bp	1.0	78.4
rpl20-rps12_p4	TTTTGTTCGACGTCTCCGAG	TCTCCCGCTCTTAGAGGATG	50.0	55.0	58.2	58.0	805-806 bp	1.0	78.1
rpl20-rps12_p5	TCCGAGCTGTATATCCTCGT	TCTCCCGCTCTTAGAGGATG	50.0	55.0	57.4	58.0	791-792 bp	1.0	75.5