Hotspot

atpH-atpI

LSC · 58178-59175 · 998 bp

Back to Wallisia

Pi 0

MarkerSeek score 59.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 998
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	1050-1055 bp	1.0	78.7
atpH-atpI_p2	CAGTACCTTGACCAACTCCG	CGAATCCATGGAGGGTCATC	55.0	55.0	57.9	57.8	1109-1114 bp	1.0	78.3
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	CGAATCCATGGAGGGTCATC	45.0	55.0	57.9	57.8	1066-1071 bp	1.0	77.8
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.2	57.8	1051-1056 bp	1.0	77.5
atpH-atpI_p5	GAAGCAAGTCCTACAGCCAA	CGAATCCATGGAGGGTCATC	50.0	55.0	57.8	57.8	1080-1085 bp	1.0	77.5