Hotspot

psbA-trnK-UUU

LSC · 45871-46100 · 230 bp

Back to Wallisia

Pi 0.0087

MarkerSeek score 57.50

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 230
variable_sites: 2
pi: 0.0087
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TAGGCTTGTACTTTCGCGTC	TGGGGAGGGATCTTTTTCCT	50.0	50.0	58.0	57.9	356 bp	1.0	79.7
psbA-trnK-UUU_p2	AACACCGAACCATCCAATGT	TGGGGAGGGATCTTTTTCCT	45.0	50.0	57.7	57.9	422 bp	1.0	77.5
psbA-trnK-UUU_p3	GCTTGTACTTTCGCGTCTCT	TGGGGAGGGATCTTTTTCCT	50.0	50.0	58.3	57.9	353 bp	1.0	77.4
psbA-trnK-UUU_p4	TCAAAACACCGAACCATCCA	TGGGGAGGGATCTTTTTCCT	45.0	50.0	57.6	57.9	426 bp	1.0	77.0
psbA-trnK-UUU_p5	TAGGCTTGTACTTTCGCGTC	AAAATGCAAGCACGGTTTGG	50.0	45.0	58.0	58.4	373 bp	1.0	77.0