Hotspot

ycf1

SSC · 128205-133838 · 5634 bp

Back to Wallichia

Pi 0.00284

MarkerSeek score 49.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5634
variable_sites: 16
pi: 0.00284
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGGAAGAACGGGGGTTTTAG	TCGACATTGAGACCTGGACT	55.0	50.0	58.2	58.1	226 bp	1.0	80.2
ycf1_p2	AAATTCTGGGAAGAACGGGG	TCGACATTGAGACCTGGACT	50.0	50.0	57.8	58.1	233 bp	1.0	79.7
ycf1_p3	AAGTGGCACAAATTCTGGGA	TCGACATTGAGACCTGGACT	45.0	50.0	57.6	58.1	242 bp	1.0	78.3
ycf1_p4	AGTGGCACAAATTCTGGGAA	TCGACATTGAGACCTGGACT	45.0	50.0	57.6	58.1	241 bp	1.0	78.3
ycf1_p5	TGGCACAAATTCTGGGAAGA	TCGACATTGAGACCTGGACT	45.0	50.0	57.3	58.1	239 bp	1.0	76.0