Hotspot

ndhH-rps15

SSC · 127481-127600 · 120 bp

Back to Wallichia

Pi 0.0171

MarkerSeek score 64.70

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: SSC
length_bp: 120
variable_sites: 2
pi: 0.0171
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	AACACCATGCATTGATGGGT	CAGTTGGATATTCGGGAGCC	45.0	55.0	58.1	58.1	225-228 bp	1.0	80.4
ndhH-rps15_p2	AACACCATGCATTGATGGGT	GGCTCCTGGCTTATTTGTCA	45.0	50.0	58.1	57.9	281-284 bp	1.0	79.9
ndhH-rps15_p3	TCAGTCGAAGAACACCATGC	CAGTTGGATATTCGGGAGCC	50.0	55.0	58.2	58.1	235-238 bp	1.0	79.1
ndhH-rps15_p4	ATGCATTGATGGGTGGTGAG	CAGTTGGATATTCGGGAGCC	50.0	55.0	58.2	58.1	219-222 bp	1.0	79.1
ndhH-rps15_p5	TCAGTCGAAGAACACCATGC	GGCTCCTGGCTTATTTGTCA	50.0	50.0	58.2	57.9	291-294 bp	1.0	78.7