Hotspot

rpl32-trnL-UAG

SSC · 117428-118199 · 772 bp

Back to Wallichia

Pi 0
MarkerSeek score 62.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
772
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGTTTCTACCGGACATTCAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 55.4 57.9 847-875 bp 1.0 60.7
rpl32-trnL-UAG_p2 GGTTTCTACCGGACATTCAA AGCAGCGTGTCTACCAATTT 45.0 45.0 55.4 57.8 840-868 bp 1.0 60.3
rpl32-trnL-UAG_p3 GGTTTCTACCGGACATTCAA CCTAAGAGCAGCGTGTCTAC 45.0 55.0 55.4 57.8 846-874 bp 1.0 60.1
rpl32-trnL-UAG_p4 GGTTTCTACCGGACATTCAA CTAAGAGCAGCGTGTCTACC 45.0 55.0 55.4 57.8 845-873 bp 1.0 60.1
rpl32-trnL-UAG_p5 GGTTTCTACCGGACATTCAA GAGCAGCGTGTCTACCAATT 45.0 50.0 55.4 58.3 841-869 bp 1.0 59.5